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Uhrf1 antibody (pAb), sample

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for Uhrf1 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

for Uhrf1 antibody (pAb)Immunogen

This Uhrf1 antibody was raised against a peptide corresponding to the N-terminal half of human Uhrf1.

for Uhrf1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Uhrf1 antibody (pAb)References

Uhrf1 antibody (pAb) tested by Western blot.

Uhrf1 pAb tested by Western blot. HEK293 nuclear extract (20 µg per lane) was probed with Uhrf1 pAb (1:500).

for Uhrf1 antibody (pAb)Background

Uhrf1 (ubiquitin-like, containing PHD and RING finger domains 1, also known as ICBP90, RNF106 or NP95) is a transcription and cell cycle regulator. It functions as a putative E3 ubiquitin-protein ligase. Uhrf1 is important for G1/S transition of the cell cycle. It is also believed that Uhrf1 may help recruit the DNA methyltransferase, DNMT1, to hemimethylated DNA and assist in the stabilization of DNMT1 with chromatin.

for Uhrf1 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Uhrf1 antibody.

for Uhrf1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Uhrf1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting