STAT3 phospho Ser727 antibody (pAb)
for STAT3 phospho Ser727 antibody (pAb)Application Notes
WB: 1:1,000 - 1:5,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
for STAT3 phospho Ser727 antibody (pAb)Immunogen
This STAT3 phospho Ser727 antibody was raised against a peptide containing phospho Ser727 of human STAT3.
for STAT3 phospho Ser727 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for STAT3 phospho Ser727 antibody (pAb)References
STAT3 (signal transducer and activator of transcription 3) is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN?, IL-10, IL-6, IL-11, IL-12, IL-2, EGF, among others.
for STAT3 phospho Ser727 antibody (pAb)Positive Control
Active Motif's Hep G2 nuclear extract (IL-6 stimulated, 100 ng/ml) (Catalog No. 36107) can be used as a positive control for STAT3 phospho Ser727 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for STAT3 phospho Ser727 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting