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STAT3 phospho Ser727 antibody (pAb)

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for STAT3 phospho Ser727 antibody (pAb)Application Notes

Validated Applications:
WB: 1:1,000 - 1:5,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for STAT3 phospho Ser727 antibody (pAb)Immunogen

This STAT3 phospho Ser727 antibody was raised against a peptide containing phospho Ser727 of human STAT3.

for STAT3 phospho Ser727 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for STAT3 phospho Ser727 antibody (pAb)References

STAT3 phospho Ser727 antibody (pAb) tested by Western blot.

STAT3 phospho Ser727 pAb tested by Western blot.
Hep G2 nuclear extract (15 µg per lane) was probed with STAT3 phospho Ser727 pAb (1:1,000).
    Lane 1: cells stimulated with IL-6 (100 ng/ml for 15 minutes at 37°C).
    Lane 2: No treatment.

STAT3 phospho Ser727 antibody (pAb) tested by dot blot analysis.

STAT3 phospho Ser727 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of STAT3 phospho Ser727 pAb for STAT2 phospho Ser727. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Unmodified Ser727 STAT1 peptide.
    Lane 2: Phospho Ser727 STAT1 peptide.
    Lane 3: Unmodified Tyr689 STAT2 peptide.
    Lane 4: Phospho Tyr689 STAT2 peptide.
    Lane 5: Unmodified Ser727 STAT3 peptide.
    Lane 6: Phospho Ser727 STAT3 peptide.
    Lane 7: Unmodified Tyr705 STAT3 peptide.
    Lane 8: Phospho Tyr705 STAT3 peptide.
    Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide.
    Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide.
    Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide.
    Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.

for STAT3 phospho Ser727 antibody (pAb)Background

STAT3 (signal transducer and activator of transcription 3) is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN?, IL-10, IL-6, IL-11, IL-12, IL-2, EGF, among others.

for STAT3 phospho Ser727 antibody (pAb)Positive Control

Active Motif's Hep G2 nuclear extract (IL-6 stimulated, 100 ng/ml) (Catalog No. 36107) can be used as a positive control for STAT3 phospho Ser727 antibody.

for STAT3 phospho Ser727 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for STAT3 phospho Ser727 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting