STAT5A/B phospho Ser726/Ser731 antibody (pAb)
for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Application Notes
WB: 1:500 - 1:2,000 dilution
for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Immunogen
This STAT5A/B phospho Ser726/Ser731 antibody was raised against a peptide containing phospho Ser726 of human STAT5A. Human STAT5B shares the identical sequence surrounding Ser731.
for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for STAT5A/B phospho Ser726/Ser731 antibody (pAb)References
STAT5A/B (signal transducer and activator of transcription 5) is a member of the STAT family of transcription factors. STATs undergo serine and tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. The STAT5 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN?, IL-10, IL-6, IL-11, IL-12, IL-2, EGF, among others.
for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Positive Control
Active Motif's Nb2 nuclear extract (prolactin stimulated) (Catalog No. 36106) can be used as a positive control for STAT5A/B phospho Ser726/Ser731 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting