Nap1 antibody (pAb)
for Nap1 antibody (pAb)Application Notes
WB: 1:1,000 - 1:5,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
for Nap1 antibody (pAb)Immunogen
This Nap1 antibody was raised against a His-Tagged full-length protein corresponding to Drosophila Nap1.
for Nap1 antibody (pAb)Buffer
PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol. Sodium azide is highly toxic.
for Nap1 antibody (pAb)References
Nap1 (Nucleosome assembly protein 1) is a member of the nucleosome assembly protein (NAP) family. Nap1 serves as a chaperone for Histone H2A-Histone H2B dimers, and participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Nap1 binds the HIV Rev protein, stimulating its RNA export function, while causing it to localize away from the nucleolus.
for Nap1 antibody (pAb)Positive Control
Active Motif's Schneider's Drosophila L2 nuclear extract (Catalog No. 36087) can be used as a positive control for Nap1 antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Nap1 antibody (pAb).
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Nap1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting