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PARP-1 N-terminal antibody (pAb), sample

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for PARP-1 N-terminal antibody (pAb)Application Notes

Validated Applications:
IP: 10 µg per IP
WB: 0.2 - 1 µg/ml dilution

for PARP-1 N-terminal antibody (pAb)Immunogen

This antibody was raised against a His-Tagged fusion protein corresponding to the N-terminal half of human PARP-1.

for PARP-1 N-terminal antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a sera version (Catalog No. 39559) of this antibody is also available.

for PARP-1 N-terminal antibody (pAb)References

PARP-1 N-terminal antibody (pAb) tested by immunoprecipitation.

PARP-1 N-terminal antibody (pAb) tested by Immunoprecipitation.
10 µg of PARP-1 antibody was used to immunoprecipitate PARP-1 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µg of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the PARP-1 antibody at a dilution of 1 µg/ml.

PARP-1 N-terminal antibody (pAb) tested by Western blot.

PARP-1 N-terminal antibody (pAb) tested by Western blot.
HeLa nuclear extract (25 µg) was probed with PARP-1 N-terminal antibody at a dilution of 1 µg/ml.

for PARP-1 N-terminal antibody (pAb)Background

PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis.

For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section of our website.

for PARP-1 N-terminal antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PARP-1 N-terminal antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting