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HMG-1 antibody (pAb)

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for HMG-1 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

Individual optimization may be required.

for HMG-1 antibody (pAb)Immunogen

This HMG-1 antibody was raised against a synthetic peptide corresponding to amino acid residue(s) 167-182 of human HMG-1.

for HMG-1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for HMG-1 antibody (pAb)References

HMG-1 antibody (pAb) tested by Western blot.

HMG-1 pAb tested by Western blot.
Detection of trimethylated HMG-1 by Western blot. The analysis was performed using 20 µg HeLa acid extract and HMG-1 pAb at a 1:2,000 dilution.

for HMG-1 antibody (pAb)Background

High mobility group proteins (HMG) are non-histone chromatin-associated proteins involved in DNA organization or regulation of transcription. Constitutively expressed in the nucleus of eukaryotic cells, HMGs share functional motifs that bind specific DNA structures (minor grooves) and induce conformational changes without specificity for target sequences. A Class II HMG member, HMG-1 binds to bent or distorted DNA such as four-way DNA junctions, synthetic DNA cruciforms, kinked cisplatin-modified DNA, DNA bulges and cross-overs in supercoiled DNA. It can cause looping of linear DNA, thus functioning as an architectural element that facilitates interactions of neighboring proteins and DNA by creating favorable DNA conformations. Extremely versatile, HMG-1 and HMG-2 are involved in numerous DNA events in the nucleus such as DNA repair, replication, recombination and transcription. Its nuclear localization and affinity for DNA is regulated through phosphorylation and acetylation, and has been found to have a dynamic relationship with chromatin. HMG-1 has been shown to stimulate the binding of a variety of sequence-specific DNA-binding proteins such as p53 in vitro, playing an important role in DNA architecture and transcriptional regulation.

for HMG-1 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for HMG-1 antibody.

for HMG-1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HMG-1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting