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alpha Tubulin antibody (mAb)

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for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Application Notes

Validated Applications:
WB: 1:500 - 1:2,500 dilution
ICC/IF: 1:1,000 - 1:10,000 dilution

for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Immunogen

This alpha Tubulin antibody was raised against total protein purified from sea urchin sperm axonemes.

for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Buffer

Ascites containing 30% glycerol and 0.035% sodium azide.

for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)References

alpha Tubulin antibody (mAb) tested by immunofluorescence.

alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence.
Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain™ Immunofluorescence Tools.

alpha Tubulin antibody (mAb) tested by Western blot.

alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot.
HeLa whole-cell extract (15 µg/lane) probed with alpha Tubulin mAb (1:5,000 dilution).

for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of ?-tubulin and ?-tubulin. Alpha Tubulin expression is reduced in cells deficient in MeCP2, leading to conditions such as Rett syndrome and autistic-spectrum disorders.

for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for alpha Tubulin antibody (Clone 5-B-1-2).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with alpha Tubulin antibody (mAb).

for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting