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MAD2L1 antibody (mAb), sample

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for MAD2L1 antibody (mAb) (Clone 17D10)Application Notes

Validated Applications:
WB: 2 - 5 µg/ml dilution

for MAD2L1 antibody (mAb) (Clone 17D10)Immunogen

This MAD2L1 antibody was raised against full-length recombinant human MAD2L1.

for MAD2L1 antibody (mAb) (Clone 17D10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MAD2L1 antibody (mAb) (Clone 17D10)References

MAD2L1 antibody (mAb) tested by Western blot.

MAD2L1 mAb (Clone 17D10) tested by Western blot.
MAD2L1 detection by Western blot. The analysis was performed using 20 µg HEK293 whole-cell extract and MAD2L1 mAb (Clone 17D10) at a 2 µg/ml dilution.

for MAD2L1 antibody (mAb) (Clone 17D10)Background

MAD2L1 (aka HSMAD2, MAD2, MAD2-like 1) is the human homolog of the budding yeast MAD2, or mitotic arrest deficient 2 gene. MAD2L1 is a component of the mitotic spindle assembly checkpoint, which monitors the attachment of the mitotic spindle to the kinetochores of segregating chromosomes and delays the onset of anaphase until attachment is complete and the chromosomes are properly aligned on the metaphase plate. MAD2L1 associates only with unattached kinetochores in prometaphase, and prevents cell cycle progression by associating with the Anaphase Promoting Complex (APC), specifically restricting the activity of the CDC20 protein.

for MAD2L1 antibody (mAb) (Clone 17D10)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for MAD2L1 antibody (Clone 17D10).

for MAD2L1 antibody (mAb) (Clone 17D10)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MAD2L1 antibody (mAb) (Clone 17D10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting