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NF-1A antibody (pAb)

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for NF-1A antibody (pAb)Application Notes

Validated Applications:
ICC/IF: 1:250 - 1:500 dilution
WB: 1:1,000 - 1:5,000 dilution

Published Applications:

for NF-1A antibody (pAb)Immunogen

This NF-1A antibody was raised against a synthetic peptide corresponding to amino acid residue(s) 478-492 of human NF-1A.

for NF-1A antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NF-1A antibody (pAb)References

NF-1A antibody (pAb) tested by immunofluorescence.

NF-1A antibody (pAb) tested by immunofluorescence.
Detection of NF-1A antibody by immunofluorescence. Human Astrocytes-cerebellar (HA-c) cells were stained with NF-1A antibody at a 1:500 dilution.
Green: NF-1A antibody (pAb)
Red: Vimentin

for NF-1A antibody (pAb)Background

NF-1A (NF-1, Nuclear factor-1) is a ubiquitous 47 kDa dimeric DNA-binding protein whose recognition sequence, TGG(C/A)N(5)GCCAA, is found in the genomes of a number of DNA viruses. The same sequence occurs frequently in the human genome. The NF-1 protein stimulates initiation of adenovirus DNA replication in vitro and is capable of stimulating the transcription of genes in cooperation with other factors, such as the estrogen receptor. The Nuclear factor-1 (NF-1) family of transcription factors are encoded by the genes NF-1A, NF-1B2, NF-1C and NF-1X and feature a highly conserved N-terminal DNA binding region and a variable C-terminal transcriptional activation or repression domain. NF-1 binding controls the expression of many target genes, including insulin, TGF-?, nucleotide, steroid hormone and vitamin synthetic genes, TNF? and FSH. NF-1 transcripts can be differentially spliced and the resultant protein variants homo- and heterodimerize, allowing for multiple potential transcriptional activation and repression activities.

for NF-1A antibody (pAb)Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for NF-1A antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with NF-1A antibody (pAb).

for NF-1A antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NF-1A antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting