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Rad21 antibody (mAb)

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for Rad21 antibody (mAb) (Clone 52A311)Application Notes

Validated Applications:
ICC/IF: 0.5 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

for Rad21 antibody (mAb) (Clone 52A311)Immunogen

This Rad21 antibody was raised against recombinant protein corresponding to the N-terminal half of mouse Rad21.

for Rad21 antibody (mAb) (Clone 52A311)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Rad21 antibody (mAb) (Clone 52A311)References

Rad21 antibody (mAb) tested by immunofluorescence.

Rad21 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with Rad21 antibody at a 0.5 µg/ml dilution.

Rad21 antibody (mAb) tested by Western blot.

Rad21 antibody (mAb) tested by Western blot.
HeLa nuclear extract (30 µg per lane) was probed with Rad21 mAb (Clone 52A311) (1 µg/ml).

for Rad21 antibody (mAb) (Clone 52A311)Background

Rad21 Clone 52A311 (HR21, MCD1, NXP1, hHR21) is highly similar to the gene product of S. pombe Rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. Rad21 is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of Rad21 with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

for Rad21 antibody (mAb) (Clone 52A311)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Rad21 antibody (Clone 52A311).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Rad21 antibody (mAb).

for Rad21 antibody (mAb) (Clone 52A311)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Rad21 antibody (mAb) (Clone 52A311)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting