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kaiso_antibody_(mab),_sample

Kaiso antibody (mAb), sample

by Active Motif
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for Kaiso antibody (mAb) (Clone 6F1D)Application Notes


Validated Applications:
WB: 0.5 - 4 µg/ml dilution

for Kaiso antibody (mAb) (Clone 6F1D)Immunogen

This Kaiso antibody was raised against a His-Tagged fusion protein corresponding to amino acids 103-499 of mouse Kaiso.

for Kaiso antibody (mAb) (Clone 6F1D)Buffer

PBS containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Kaiso antibody (mAb) (Clone 6F1D)References

 
Kaiso antibody (mAb) tested by Western blot.

Kaiso mAb (Clone 6F1D) tested by Western blot.
Nuclear extract of CSML0 cells (20 µg) was probed with Kaiso mAb (Clone 6F1D) (1 µg/ml dilution).

for Kaiso antibody (mAb) (Clone 6F1D)Background

Kaiso (Clone 6F1D) is a zinc-finger transcription factor protein that binds to methylated and non-methylated GTGCNA motifs, and is also known as ZNF-Kaiso or ZBTB33. Kaiso interacts with the cell adhesion cofactor p120 catenin and may also recruit N-CoR repressor complex to promote histone deacetylation and repress transcriptional activity.

for Kaiso antibody (mAb) (Clone 6F1D)Positive Control

Nuclear extracts form CSML0 cells can be used as a positive control for Kaiso antibody (Clone 6F1D).

for Kaiso antibody (mAb) (Clone 6F1D)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Kaiso antibody (mAb) (Clone 6F1D)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting