Mi-2 beta antibody (pAb), sample
for Mi-2 beta antibody (pAb)Application Notes
ICC/IF: 1:500 - 1:2,000 dilution
WB: 1:500 - 1:1,000 dilution
See references for more information. Individual optimization may be required.
for Mi-2 beta antibody (pAb)Immunogen
This Mi-2B antibody was raised against a recombinant protein corresponding to amino acids 22-155 of mouse Mi-2 beta.
for Mi-2 beta antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for Mi-2 beta antibody (pAb)References
Mi-2? (Mi-2beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).
for Mi-2 beta antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Mi-2 beta antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Mi-2 beta antibody (pAb).
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Mi-2 beta antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting