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Lamin A/C antibody (mAb)

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for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Application Notes

Validated Applications:
WB: 0.5 - 2 µg/ml dilution
ICC/IF: 2 - 5 µg/ml dilution

Published Applications: see references

Both lamin A and lamin C are recognized by this sera, with lamin A (the slower migrating band on Western blot) sometimes appearing as a doublet. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. For immunofluorescence experiments we recommend using paraformaldehyde (PFA) fixation.

for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Immunogen

This Lamin A/C antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A.

for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Buffer

Cell culture supernatant containing mouse IgG, 30% glycerol, 0.035% sodium azide.

Also available as an AbFlex™ engineered recombinant antibody.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91189.

for Lamin A/C antibody (mAb) (Clone 3A6-4C11)References

Lamin A/C antibody (mAb) tested by immunofluorescence.

Lamin A/C mAb (Clone 3A6-4C11) tested by immunofluorescence.
Staining of HeLa cells with Lamin A/C mAb (Clone 3A6-4C11). Cells were fixed with 3.7% PFA for 15 minutes at room temperature, permeabilized with PBS + 0.5% Triton™ X-100 and stained with Lamin A/C mAb (2 µg/ml dilution) followed by 1:500 dilution Chromeo™ 546 Goat anti-mouse IgG (Cat No. 15033) (top panel) and DAPI (bottom panel).

Lamin A/C antibody (mAb) tested by Western blot.

Lamin A/C mAb (Clone 3A6-4C11) tested by Western blot.
Whole-cell extract (20 µg) of HeLa cells stained with Lamin A/C mAb (Clone 3A6-4C11) at a dilution of 0.5 µg/ml.

for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Background

Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.

for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Lamin A/C antibody (Clone 3A6-4C11).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Lamin A/C antibody (mAb).

for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting