Myc-Tag antibody (mAb), sample
for Myc-Tag antibody (mAb) (Clone 4E12)Application Notes
WB: 0.5 - 2 µg/ml dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
for Myc-Tag antibody (mAb) (Clone 4E12)Immunogen
This Myc-Tag antibody was raised against peptide corresponding to amino acids 410-419 of human c-Myc (EQKLISEEDL).
for Myc-Tag antibody (mAb) (Clone 4E12)Buffer
Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for Myc-Tag antibody (mAb) (Clone 4E12)References
Myc-Tag is a sequence derived from the human c-Myc protein that has been developed into an effective epitope for detection of tagged proteins expressed in vivo. Clone 4E12 exhibits superior detection of Myc-Tagged proteins compared to the more common 9E10 clone.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Myc-Tag antibody (mAb) (Clone 4E12)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting