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Myc-Tag antibody (mAb)

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for Myc-Tag antibody (mAb) (Clone 4E12)Application Notes

Validated Applications:
WB: 0.5 - 2 µg/ml dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for Myc-Tag antibody (mAb) (Clone 4E12)Immunogen

This Myc-Tag antibody was raised against peptide corresponding to amino acids 410-419 of human c-Myc (EQKLISEEDL).

for Myc-Tag antibody (mAb) (Clone 4E12)Buffer

Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Myc-Tag antibody (mAb) (Clone 4E12)References

Myc-Tag antibody (mAb) tested by Western blot.

Myc-Tag mAb tested by Western blot.
Whole-cell extract (20 µg) of NIH/3T3 cells expressing a Myc-Tagged PP2A-alpha subunit blotted with Myc-Tag mAb (0.5 µg/ml).

for Myc-Tag antibody (mAb) (Clone 4E12)Background

Myc-Tag is a sequence derived from the human c-Myc protein that has been developed into an effective epitope for detection of tagged proteins expressed in vivo. Clone 4E12 exhibits superior detection of Myc-Tagged proteins compared to the more common 9E10 clone.

for Myc-Tag antibody (mAb) (Clone 4E12)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Myc-Tag antibody (mAb) (Clone 4E12)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting