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Cyk-4 phospho Ser170 antibody (pAb)

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for Cyk-4 phospho Ser170 antibody (pAb)Application Notes

Validated Applications:
ICC/IF: 1:200 - 1:500 dilution
WB: 1:1,000 - 1:2,500 dilution

This Cyk-4 antibody must first be immunoprecipitated and then run on a gel before Western blotting.

for Cyk-4 phospho Ser170 antibody (pAb)Immunogen

This Cyk-4 phospho Ser170 antibody was raised against a peptide containing phospho Ser170 of human Cyk-4.

for Cyk-4 phospho Ser170 antibody (pAb)Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Cyk-4 phospho Ser170 antibody (pAb)References

Cyk-4 phospho Ser170 antibody (pAb) tested by immunofluorescence.

Cyk-4 phospho Ser170 pAb tested by immunofluorescence.
Normal human retinal pigment epithelial cells stained with 39265 (1:300). Left: 39265. Right: merge with DAPI.

Cyk-4 phospho Ser170 antibody (pAb) tested by Western blot.

Cyk-4 phospho Ser170 Rabbit pAb tested by Western Blot.
Recombinant Cyk-4 fragment (100 ng) untreated (lane 1) and incubated with the kinase Plk-1 (lane 2) was probed with 39265 at 1:2,000.

Cyk-4 phospho Ser170 antibody (pAb) tested by dot blot analysis.

Cyk-4 phospho Ser170 pAb tested by dot blot to show specificity for serine 170.
Dot blot analysis was used to confirm the specificity of 39265 for phospho Ser170 Cyk-4. The modified and unmodified peptides to the immunogen were spotted onto PVDF and probed with 39265 at a dilution of 1:2,000. The amount of peptide (picomoles) spotted is 250,50,10 and 2 from top to bottom.

Lane 1: phospho Ser170 peptide.
Lane 2: unmodified Ser170 peptide.

for Cyk-4 phospho Ser170 antibody (pAb)Background

Cyk-4 is a member of a GTPase activating protein family that regulates the catalytic function of Rho and Ras small G-proteins. Cyk-4 regulates the initiation of cytokinesis through its interaction with a variety of microtubule organizing proteins. The activity of Cyk-4 is regulated by phophorylation of serine 170 by Polo-like Kinase 1.

Burkard, ME et al (2009) PLoS Biol. 2009 7:e1000111.

for Cyk-4 phospho Ser170 antibody (pAb)Positive Control

Recombinant Cyk-4 fragment can be used as a positive control for Cyk-4 phospho Ser170 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Cyk-4 phospho Ser170 antibody (pAb).

for Cyk-4 phospho Ser170 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Cyk-4 phospho Ser170 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting