Cyk-4 phospho Ser170 antibody (pAb)
for Cyk-4 phospho Ser170 antibody (pAb)Application Notes
ICC/IF: 1:200 - 1:500 dilution
WB: 1:1,000 - 1:2,500 dilution
This Cyk-4 antibody must first be immunoprecipitated and then run on a gel before Western blotting.
for Cyk-4 phospho Ser170 antibody (pAb)Immunogen
This Cyk-4 phospho Ser170 antibody was raised against a peptide containing phospho Ser170 of human Cyk-4.
for Cyk-4 phospho Ser170 antibody (pAb)Buffer
PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for Cyk-4 phospho Ser170 antibody (pAb)References
Cyk-4 is a member of a GTPase activating protein family that regulates the catalytic function of Rho and Ras small G-proteins. Cyk-4 regulates the initiation of cytokinesis through its interaction with a variety of microtubule organizing proteins. The activity of Cyk-4 is regulated by phophorylation of serine 170 by Polo-like Kinase 1.
Burkard, ME et al (2009) PLoS Biol. 2009 7:e1000111.
for Cyk-4 phospho Ser170 antibody (pAb)Positive Control
Recombinant Cyk-4 fragment can be used as a positive control for Cyk-4 phospho Ser170 antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Cyk-4 phospho Ser170 antibody (pAb).
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Cyk-4 phospho Ser170 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting