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JMJD2D antibody (pAb)

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for JMJD2D antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

for JMJD2D antibody (pAb)Immunogen

This JMJD2D antibody was raised against a peptide corresponding to amino acids 337-359 of human JMJD2D.

for JMJD2D antibody (pAb)Buffer

Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for JMJD2D antibody (pAb)References

JMJD2D antibody (pAb) tested by Western blot.

JMJD2D pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) probed with JMJD2D pAb (1:1,000). Specificity was verified by peptide competition.
    Lane 1: No peptide.
    Lane 2: 1 µM immunizing peptide.

for JMJD2D antibody (pAb)Background

JMJD2D (KDM4D) is a histone demethylase located in the nucleus that specifically demethylates Lysine 9 of Histone H3, thereby playing a central role in the histone code. JMJD2D (KDM4D) demethylates both di- or trimethylated histone H3 Lys 9, while it has no activity on monomethylated residues. The demethylation reaction of the Lys residue generates formaldehyde and succinate as a by product. In addition, JMJD2D forms a complex with the ligand-bound form of the androgen receptor (AR) and, through this interaction, activates AR expression. Overexpression of AR is associated with prostate cancer, suggesting that, via its ability to upregulate AR, JMJD2D (KDM4D) may be involved in carcinogenesis.

for JMJD2D antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JMJD2D antibody.

for JMJD2D antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for JMJD2D antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting