for UBE2V2 antibody (pAb)Application Notes
Validated Applications:
WB: 1 - 3 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for UBE2V2 antibody (pAb)Immunogen
This UBE2V2 antibody was raised against a peptide corresponding to amino acid residues 12-28 of human UBE2V2.
for UBE2V2 antibody (pAb)Buffer
PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.
for UBE2V2 antibody (pAb)References
UBE2V2 pAb tested by Western blot.
Detection of UBE2V2 by Western blot. The analysis was performed using HL-60 whole-cell extract and UBE2V2 pAb at a 2 µg/ml dilution.
UBE2V2 – Ubiquitin-Conjugating Enzyme E2 Variant 2 is a member of the Ubiquitin-conjugating E2 family of proteins but lacks the conserved cysteine residue that is critical for the catalytic activity of E2 enzymes and has no intrinsic E2 ligase activity. The protein encoded by this gene also shares homology with ubiquitin-conjugating enzyme E2 variant 1 and the yeast MMS2 gene product. It forms a heterodimer with UBE2N and catalyzes the synthesis of poly-ubiquitin chains that are linked through Lys63, which does not lead to protein degradation by the proteasome. Instead, it mediates transcriptional activation of target genes, plays a role in the control of progress through the cell cycle as well as differentiation. It also plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
for UBE2V2 antibody (pAb)Positive Control
Active Motif's HL-60 nuclear extract (Catalog No. 36072) can be used as a positive control for UBE2V2 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for UBE2V2 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting
