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ube2n_antibody_(pab)

UBE2N antibody (pAb)

by Active Motif
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for UBE2N antibody (pAb)Application Notes


Validated Applications:
WB: 2 µg/ml dilution

For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for UBE2N antibody (pAb)Immunogen

This UBE2N antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 2-19 and 131-148 of human UBE2N.

for UBE2N antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for UBE2N antibody (pAb)References

 
UBE2N antibody (pAb) tested by Western blot.

UBE2N pAb tested by Western blot.
UBE2N detection by Western blot. The analysis was performed using human heart whole-cell extract and UBE2N pAb at a 2 µg/ml dilution.

for UBE2N antibody (pAb)Background

UBE2N (UBC13, BLU, ubiquitin-conjugating enzyme E2N) heterodimer with UBE2V2 catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBE2N mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation. UBE2N also plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage and acts together with the E3 ligase SHPRH in the poly-ubiquitination of PCNA 'Lys-164' upon genotoxic stress, which is required for DNA repair.

for UBE2N antibody (pAb)Positive Control

Active Motif's Human Heart nuclear extract (Catalog No. 36041) can be used as a positive control for UBE2N antibody.

for UBE2N antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for UBE2N antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting