UBE2N antibody (pAb)
for UBE2N antibody (pAb)Application Notes
WB: 2 µg/ml dilution
For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for UBE2N antibody (pAb)Immunogen
This UBE2N antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 2-19 and 131-148 of human UBE2N.
for UBE2N antibody (pAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for UBE2N antibody (pAb)References
UBE2N (UBC13, BLU, ubiquitin-conjugating enzyme E2N) heterodimer with UBE2V2 catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBE2N mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation. UBE2N also plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage and acts together with the E3 ligase SHPRH in the poly-ubiquitination of PCNA 'Lys-164' upon genotoxic stress, which is required for DNA repair.
for UBE2N antibody (pAb)Positive Control
Active Motif's Human Heart nuclear extract (Catalog No. 36041) can be used as a positive control for UBE2N antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for UBE2N antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting