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TRF2 antibody (pAb)

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for TRF2 antibody (pAb)Application Notes

Validated Applications:
IP: 2 µg per IP
WB: 1 - 2 µg/ml dilution

For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for TRF2 antibody (pAb)Immunogen

This TRF2 antibody was raised against a full-length protein corresponding to human TRF2.

for TRF2 antibody (pAb)Buffer

PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.

for TRF2 antibody (pAb)References

TRF2 antibody (pAb) tested by Western blot.

TRF2 pAb tested by Western blot.
Western Blot Analysis of TRF2 in HL-60 cells.
    Lane 1. IP with a mouse anti-TRF2.
    Lane 2. IP with control mouse IgG.
    Lane 3 IP with TRF2 pAb (Cat. No. 39223.)
    Lane 4. IP with pre-immune goat Ig.
    Lanes 1-4. WB with mouse anti-TRF2. TRF2 is detected as an ~ 66 kDa protein.

for TRF2 antibody (pAb)Background

TRF2 is a sequence-specific DNA binding protein that binds the duplex array of TTAGGG repeats at human telomeres. In particular, the telomere-binding protein TRF2 regulates both telomere protection and telomere length (end to end fusions are avoided through the formation of the t-loop). Telomere shortening is envisioned as a potent tumor suppressor mechanism. TRF2 is overexpressed in several human tumors, such as breast carcinomas, liver hepatocarcinomas, and lung carcinomas suggesting a general role in tumorigenesis. Removal of TRF2 results in the localization of active ATM to the unprotected telomeres, eventually inducing ATM-dependent apoptosis in telomerase-positive tumor cell lines. TRF2 has been shown to interact with ATM, through S1981 in an unphosphorylated state, and block its activation. It also interacts with several DNA repair proteins that are involved in human chromosomal instability syndromes characterized by premature aging and increased cancer. In particular, TRF2 interacts with Rap1, the WRN and BLM helicases, the DNA–PK complex, the MRE11/NBS1/Rad50 complex, PARP1 and PARP2 poly(ADP)ribosylases, and the ERCC1/XPF endonuclease.

for TRF2 antibody (pAb)Positive Control

Active Motif's HL-60 nuclear extract (Catalog No. 36072) can be used as a positive control for TRF2 antibody.

for TRF2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TRF2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting