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MBD1 antibody (mAb)

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for MBD1 antibody (mAb) (Clone 100B272.1)Application Notes

Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for MBD1 antibody (mAb) (Clone 100B272.1)Immunogen

This MBD1 antibody was raised against a synthetic peptide corresponding to human MBD1.

for MBD1 antibody (mAb) (Clone 100B272.1)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for MBD1 antibody (mAb) (Clone 100B272.1)References

MBD1 antibody (mAb) tested by Western blot.

MBD1 antibody tested by Western blot.
MBD1 detection by Western blot. The analysis was performed using HeLa whole-cell extract and MBD1 antibody at 2 µg/ml (lane 1) and a 1 µg/ml (lane 2) dilutions.

for MBD1 antibody (mAb) (Clone 100B272.1)Background

MBD1 (methyl-CpG binding domain protein 1) is thought to function as a mediator of the biological consequences of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD1 is recruited to both methylated and non-methylated CpGs via separate domains: the CXXC domain targets the protein to non-methylated CpGs whereas the MBD domain targets the protein to methylated CpG. During S phase of the cell cycle, MBD1 can interact with SETDB1 (H3K9 methyltransferase).

for MBD1 antibody (mAb) (Clone 100B272.1)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for MBD1 antibody.

for MBD1 antibody (mAb) (Clone 100B272.1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MBD1 antibody (mAb) (Clone 100B272.1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting