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cxxc1_antibody_(pab)

CXXC1 antibody (pAb)

by Active Motif
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Size

for CXXC1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for CXXC1 antibody (pAb)Immunogen

This CXXC1 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 73-90 and 641-656 of human CXXC1.

for CXXC1 antibody (pAb)Buffer

PBS containing 0.05% BSA and 0.02% sodium azide. Sodium azide is highly toxic.

for CXXC1 antibody (pAb)References

 
CXXC1 antibody (pAb) tested by Western blot.

CXXC1 pAb tested by Western blot.
Detection of CXXC1 by Western blot analysis. Western blot analysis using CXXC1 pAb at a 1:1,000 dilution against 15 µg/lane of human spleen lysate.

for CXXC1 antibody (pAb)Background

CXXC1 (also known as CGBP or CpG binding protein) is a transcriptional activator protein that binds unmethylated CpG dinucleotides and plays a role in regulating gene expression and embryonic development. CGBP exhibits a different distribution pattern than other heterochromatin-associated proteins. It seems to co-localize with acetylated histones, indicating that CXXC1 is associated with active chromatin.

for CXXC1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CXXC1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting