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Rad51 antibody (pAb)

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for Rad51 antibody (pAb)Application Notes

Validated Applications:
ICC/IF: 1 - 5 µg/ml dilution

Published Applications: see references

for Rad51 antibody (pAb)Immunogen

This Rad51 antibody was raised against full-length human Rad51 expressed in E. coli.

for Rad51 antibody (pAb)Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Rad51 antibody (pAb)References

Rad51 antibody (pAb) tested by immunofluorescence.

Rad51 pAb tested by immunofluorescence.
HEK293 cells stained with Rad51 polyclonal antibody (green) and DAPI (blue).

for Rad51 antibody (pAb)Background

Rad51 (BRCC5, RecA) is known to be involved in the homologous recombination and repair of DNA. Rad51 can interact with the ssDNA-binding protein RPA and Rad52, and it is thought to play roles in homologous pairing and strand transfer of DNA. Rad51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.

for Rad51 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Rad51 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Rad51 antibody (pAb).

for Rad51 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Rad51 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting