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MEP50 antibody (pAb)

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for MEP50 antibody (pAb)Application Notes

Validated Applications:
WB: 1:1,000 - 1:2,500 dilution

for MEP50 antibody (pAb)Immunogen

This MEP50 antibody was raised against full-length human MEP50 with an N-terminal His-Tag.

for MEP50 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MEP50 antibody (pAb)References

MEP50 antibody (pAb) tested by Western blot.

MEP50 pAb tested by Western blot.
Whole-cell extract of HeLa cells (20 µg per lane) was probed with MEP50 pAb at a dilution of 1:2,500 dilution (lane 1). In lane 2, the antibody was pre-incubated with 1 µM recombinant MEP50 protein, eliminating the band and demonstrating the specificity of MEP50 pAb for MEP50.

for MEP50 antibody (pAb)Background

MEP50 (also designated WDR77) is an important component of the methylosome, a large (20S) protein arginine methyltransferase complex that contains PRMT5. The methylosome functions to modify specific arginines to dimethylarginines in the arginine- and glycine-rich domains of several spliceosomal Sm proteins. This modification targets these proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein (snRNP) core particles. A 50 kDa WD repeat protein MEP50 binds to JBP1 and a subset of Sm proteins and is thought to provide an interface for multiple protein interactions between methylosome proteins. The ability of MEP50 to interact with multiple proteins suggests it is important for targeting the Sm as well as other yet unknown proteins to the methylosome for symmetrical dimethylation. MEP50 specifically interacts with CTD phosphatase FCP1 (CTDP1), a protein that may link the processes of transcriptional elongation and splicing, and with Suz12, a polycomb group protein involved in transcriptional repression.

for MEP50 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for MEP50 antibody.

for MEP50 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MEP50 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting