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3T3-L1 nuclear extract

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for 3T3-L1 nuclear extractContents

200 µg of 3T3-L1 nuclear extract at 5.18 µg/µl.

for 3T3-L1 nuclear extractBackground

3T3-L1 nuclear extract was prepared from cell cultures of the mouse embryonic preadipocyte 3T3-L1 cell line. This cell line is derived from the original 3T3-Swiss albino cell line and has a fibroblast-like morphology. However, under appropriate conditions, 3T3-L1 cells are capable of differentiating into an adipocyte-like phenotype and are responsive to lipogenic and lipolytic hormones and drugs. These cells are commonly used as an in vitro model of adipocytes and in studies related to obesity and metabolic diseases.

for 3T3-L1 nuclear extractApplication Notes

3T3-L1 nuclear extract is specifically recommended for studies of 1) adipocyte differentiation, 2) obesity, 3) diabetes, and 4) cardiovascular disease and 5) STATs 1, 3, 5A, 5B and 6.

for 3T3-L1 nuclear extractExtract Origin

Murine Embryo Fibroblast

for 3T3-L1 nuclear extractExtract Composition

Nuclear extracts from 3T3-L1 cells are supplied from embryonic adipose 3T3 cells and are supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

for 3T3-L1 nuclear extractQuality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

for 3T3-L1 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for 3T3-L1 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.