293 nuclear extract
for 293 nuclear extractContents
200 µg of 293 nuclear extract at 4.68 µg/µl.
for 293 nuclear extractBackground
293 nuclear extract was prepared from cell cultures of the Human Embryonic Kidney (HEK) 293 cell line. This cell line was originally derived from transformation of normal human embryonic tissue cultures with a sheared DNA fragment of the left arm of the adenoviral 5 viral genome. These cells are commonly used in research due to their ease of growth and transfectability. However, since the cellular origin of these hypotriploid cells is poorly understood, they are not recommended for use as an in vitro model for kidney cell function. 293 nuclear extract contains all the basal transcriptional and mRNA splicing components and serves as an ideal cell-free platform for analysis of transcription and pre-mRNA processing mechanisms.
for 293 nuclear extractApplication Notes
293 nuclear extract is specifically recommended for analysis of 1) in vitro transcription, 2) protein-protein and protein-DNA/RNA interactions, and 3) in vitro splicing.
for 293 nuclear extractExtract Origin
Human Embryonic Kidney
for 293 nuclear extractExtract Composition
293 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
for 293 nuclear extractQuality Control
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
for 293 nuclear extractGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.