Recombinant pRb protein
for Recombinant pRb proteinContents
5 µg of Recombinant pRb protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Please refer to product insert upon arrival for lot-specific concentration.
for Recombinant pRb proteinApplication Notes
Recombinant pRb is suitable for protein-protein interaction, in vitro function studies including transcription and DNA binding assays and cell growth assays. 1-5 ng is sufficient for gelshift assays in a 20 µl volume, 50 ng is sufficient for reconstituted transcription assays and 100 ng is sufficient for protein-protein interaction assays. The molecular weight of the protein is ~105 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
for Recombinant pRb proteinProtein Details
The wild-type pRb protein was expressed in a baculovirus system (accession number NM 000321) with an N-terminal His-Tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.