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3-Methylcytosine (3-mC) antibody (pAb)

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for 3-Methylcytosine (3-mC) antibody (pAb)Application Notes

Validated Applications:
DB: 0.1 - 1 µg/ml dilution

Published Applications:
See references for more information. Individual optimization may be required.

for 3-Methylcytosine (3-mC) antibody (pAb)Immunogen

This 3-methylcytosine antibody was raised against 3-methylcytidine conjugated to KLH and recognizes 3-methylcytosine.

for 3-Methylcytosine (3-mC) antibody (pAb)Buffer

Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for 3-Methylcytosine (3-mC) antibody (pAb)References

3-Methylcytosine (3-mC) antibody (pAb) tested by dot blot analysis.

3-Methylcytosine (3-mC, 3-methylcytidine) antibody tested by dot blot analysis.
BSA conjugated nucleosides (starting at 10ng as indicated) were spotted onto PVDF membrane and blotted with 3-methylcytidine antibody at a dilution of 1:10,000.
Lane 1: 3-methylcytidine.
Lane 2: Cytidine.
Lane 3: 5-methylcytidine.
Lane 4: 5-hydroxymethylcytidine.

for 3-Methylcytosine (3-mC) antibody (pAb)Background

Methylation of DNA can occur non-enzymatically at the nitrogen-three of the cytosine base through spontaneous exposure to endogenous S-adenosyl methionine (SAM). The resulting 3-methylcytosine (3-mC) is mutagenic and must be repaired, which occurs in humans through the base excision repair (BER) or dealkylation via human homologues of the E. coli AlkB protein. 3-methylcytosine is present in human cell lines and increased levels of 3-mC impair proliferation.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with 3-Methylcytosine (3-mC) antibody (pAb).

for 3-Methylcytosine (3-mC) antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for 3-Methylcytosine (3-mC) antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting