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BrdU antibody (mAb)

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for BrdU antibody (mAb) (Clone Bu20a)Application Notes

Published Applications:
See references for more information. Individual optimization may be required.

Also available as an AbFlex® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91269.

for BrdU antibody (mAb) (Clone Bu20a)Immunogen

This BrdU antibody was raised against Bromodeoxyuridine conjugated to BSA and recognizes BrdU.

for BrdU antibody (mAb) (Clone Bu20a)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for BrdU antibody (mAb) (Clone Bu20a)References

for BrdU antibody (mAb) (Clone Bu20a)Background

BrdU (Bromodeoxyuridine) is a thymidine analogue, capable of being incorporated into DNA in place of thymidine during DNA synthesis. By pulse labelling with BrdU, the DNA synthesis in the cells can be analysed by detection with anti-BrdU antibodies. Antibodies against BrdU recognize BrdU in single-stranded DNA (produced by partial denaturation of double-stranded DNA), as well as free BrdU or BrdU coupled to a protein carrier.

for BrdU antibody (mAb) (Clone Bu20a)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for BrdU antibody (mAb) (Clone Bu20a)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting