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HIF-1 alpha antibody (mAb)

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for HIF-1 alpha antibody (mAb) (Clone ESEE122)Application Notes

Published Applications:

See references for more information. Individual optimization may be required.

for HIF-1 alpha antibody (mAb) (Clone ESEE122)Immunogen

This HIF-1 alpha antibody was raised against a partial protein containing amino acid residues 329-530 of human HIF-1 alpha.

for HIF-1 alpha antibody (mAb) (Clone ESEE122)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Also available as AbFlex™ engineered recombinant antibodies.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91139.

for HIF-1 alpha antibody (mAb) (Clone ESEE122)References

for HIF-1 alpha antibody (mAb) (Clone ESEE122)Background

HIF-1 (hypoxia inducible factor 1, alpha subunit, MOP1, PASD8, bHLHe78, PAS domain-containing protein 8) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

for HIF-1 alpha antibody (mAb) (Clone ESEE122)Positive Control

Active Motif's COS-7 (CoCl2 treated) nuclear extract (Catalog No. 40600) can be used as a positive control for HIF-1 alpha.

for HIF-1 alpha antibody (mAb) (Clone ESEE122)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HIF-1 alpha antibody (mAb) (Clone ESEE122)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting