Histone H3K4K9K18 (pan-biotinylated) antibody (pAb), sample
for Histone H3K4K9K18 (pan-biotinylated) antibody (pAb)Application Notes
See references for more information. Individual optimization may be required.
For Western blotting, antibody should be incubated overnight at 4°C with 0.5-0.67M NaCl containing PBS with 0.05% Tween 20. For diluting secondary antibodies use a 0.237M NaCl containing PBS with 0.05% Tween 20. In addition, washing steps should be performed with 0.5-0.67M NaCl containing PBS with 0.05% Tween 20.
for Histone H3K4K9K18 (pan-biotinylated) antibody (pAb)Immunogen
This antibody was raised against a synthetic peptide containing biotin-Lys 4 of human Histone H3.
for Histone H3K4K9K18 (pan-biotinylated) antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3K4K9K18 (pan-biotinylated) antibody (pAb)References
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.
Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation, SUMOylation and biotinylation; these modifications play a major role in regulating gene expression.
The biotinylation of Histone H3 on Lys4, Lys9 and Lys18 has been reported to be enriched in repeat regions and may participate in repression of transcriptionally competent genes.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K4K9K18 (pan-biotinylated) antibody (pAb).
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Histone H3K4K9K18 (pan-biotinylated) antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting