ADP-Glo Kinase Assay + Aurora A Kinase Enzyme System
Recombinant full-length human Aurora A was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. Aurora A belongs to a multigenic family of mitotic serine/threonine kinases, which are involved in the control of chromosome segregation. Aurora A is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. Aurora A is expressed and active at the highest level during G2-M phase of the cell cycle. Overexpression of Aurora A has been found to be correlated with the grade of various human solid tumors. Ectopic Aurora A overexpression in any culture cell line leads to polyploidy and centrosome amplification. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: Aurora A, 10ug (Human, recombinant full-length). MW: ~72kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. Aurora A NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/6790/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.