ADP-Glo Kinase Assay + CAMK2 alpha Kinase Enzyme System
Recombinant full-length human CAMK2 alpha was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 alpha is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family. CAMK2 alpha is abundant in the brain as a major constituent of the postsynaptic density and is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its Ca2+/calmodulin-dependent activity, CAMK2 alpha can undergo autophosphorylation, resulting in Ca2+/calmodulin-independent activity. The protein level of CAMK2 alpha fluctuates during neuronal activity in cultured rat pup hippocampal neurons. The levels of CAMK2 alpha increased with heightened neuronal activity (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: CAMK2 alpha, 10ug (Human, recombinant full-length). MW: ~74kDa. Substrate: Autocamtide 2 peptide (KKALRRQETVDAL-amide); derived from the autophosphorylation site (amino acids 283-290) on CaMKII. Other: Reaction Buffer, DTT, Ca2+/Calmodulin solution. CAMK2 alpha NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/815/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.