ADP-Glo Kinase Assay + ERK1 Kinase Enzyme System
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs), which are activated in response to numerous growth factors and cytokines. Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK1 translocates into the nucleus, where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos and C/EBP beta). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: ERK1, 10ug (Human, recombinant full-length). MW: ~44kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. ERK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5595/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.