ADP-Glo Kinase Assay + HER2 Kinase Enzyme System
Recombinant human HER2 (amino acids 676-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to the epidermal growth factor receptor. HER2 is an oncogene, and overexpression of unaltered HER2 coding sequences in NIH/3T3 cells results in cellular transformation and tumorigenesis. HER2 is amplified in about 30% of primary human breast malignancies, and overexpression of HER2 is associated with the most aggressive tumors that show uncontrolled proliferation, resistance to apoptosis and increased motility. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: HER2, 10ug (Human, recombinant; amino acids 676-end). MW: ~116kDa. Substrate: Poly (4:1 Glu, Tyr) Peptide. Other: Reaction Buffer, DTT. HER2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/2064/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.