ADP-Glo Kinase Assay + JNK1 Kinase Enzyme System
Recombinant full-length human JNK1 (sequence matches mouse 100% in protein sequence) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at the thr and tyr residues during exposure to stress such as UV irradiation. JNK1 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK1 has been shown to play an important role in disease processes. Activation of JNK1 results in defects in myotube viability and integrity leading to dystrophic myofiber destruction (2). JNK1 activity also is abnormally elevated in obesity, and removal of JNK1 results in decreased adiposity and significantly improved insulin sensitivity. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: JNK1, 10ug (Human, recombinant full-length). MW: ~70kDa. Substrate: p38 substrate (IPTTPITTTYFFFKKK). Other: Reaction Buffer, DTT. JNK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/26419/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.