ADP-Glo Kinase Assay + MARK1 Kinase Enzyme System
Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction. Due to its protein serine/threonine kinase activity, MARK1 is known to mediate phosphorylation of key proteins involved in signal transduction and cell communication. MARK1 phosphorylates microtubule-associated proteins and triggers microtubule disruption (1). Gene mutation studies performed in mice revealed that, after targeted disruption of the MARK1 gene, the mice lacked the ability to drink and displayed hind leg motor dysfunction (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: MARK1, 10ug (Human, recombinant full-length). MW: ~125kDa. Substrate: Chktide (KKKVSRSGLYRSPSMPENLNRPR); derived from human CDC25C protein isoform A (amino acids 205-225). Other: Reaction Buffer, DTT. MARK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/4139/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.