ADP-Glo Kinase Assay + NIK Kinase Enzyme System

Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 and stimulates NF-kappaB activity. NIK shares sequence similarity with several other MAPKK kinases and participates in NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor (1). NIK is expressed in primary human cells and inflamed rheumatoid arthritis tissue and plays a selective role in signaling by the lymphotoxin-beta receptor (2). NIK is a therapeutic target in the immune and bone-destructive components of inflammatory arthritis. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: NIK, 10ug (Human, recombinant; amino acids 325-end). MW: ~108kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. NIK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/9020/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.