ADP-Glo Kinase Assay + p38 beta Kinase Enzyme System
Recombinant full-length human p38 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38 beta is a member of the p38 MAP kinase family and is activated by both proinflammatory cytokines and environmental stress (1). The p38 beta is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase (2). Alternatively spliced transcript variants encoding the same protein have been observed. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: p38 beta, 10ug (Human, recombinant full-length). MW: ~71kDa. Substrate: p38 substrate (IPTTPITTTYFFFKKK). Other: Reaction Buffer, DTT. p38 beta NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5600/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.