ADP-Glo Kinase Assay + p38 delta Kinase Enzyme System
Recombinant full-length human p38 delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38 delta (SAPK4) is a member of the p38 MAPK family and is activated by chemical and environmental stresses as well as by proinflammatory cytokines. p38 delta has a TGY dual phosphorylation motif and is activated in response to cellular stresses and proinflammatory cytokines (1). MAP kinase kinases 3 and 6 can phosphorylate and activate this kinase. Transcription factor ATF2 and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: p38 delta, 10ug (Human, recombinant full-length). MW: ~71kDa. Substrate: p38 substrate (IPTTPITTTYFFFKKK). Other: Reaction Buffer, DTT. p38 delta NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5603/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.