ADP-Glo Kinase Assay + PKC epsilon Kinase Enzyme System
Recombinant full-length human PKC epsilon was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC epsilon is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKC epsilon is involved in many different cellular functions, such as neuron channel activation, cardioprotection from ischemia (1), heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may control anxiety-like behavior (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: PKC epsilon, 10ug (Human, recombinant full-length). MW: ~110kDa. Substrate: PKCtide (ERMRPRKRQGSVRRRV); derived from protein kinase C epsilon (amino acids 149-164). Other: Reaction Buffer, DTT, Lipid Solution. PKC epsilon NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5581/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.