ADP-Glo Kinase Assay + SRC Kinase Enzyme System
Recombinant full-length human SRC was expressed in E. coli cells using an N-terminal GST tag. SRC family belongs to nonreceptor tyrosine kinases. SRC was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates. SRC is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases. In addition to increasing cell proliferation, a key role of SRC in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumor progression. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: SRC, 10ug (Human, recombinant full-length). MW: ~83kDa. Substrate: SRC substrate (KVEKIGEGTYGVVYK-amide); derived from human p34cdc2 (amino acids 6-20). Other: Reaction Buffer, DTT, MnCl2. SRC NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/6714/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.