ADP-Glo Kinase Assay + TNIK Kinase Enzyme System

Recombinant human TNIK (1-367) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TNIK or TRAF2 and NCK-interacting kinase are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serve a regulatory function (1). TNIK is mainly expression in brain, heart and spleen and is a specific effector of RAP2, which regulates actin cytoskeleton (2). TNIK is autophosphorylated in a manner dependent upon Lys54 in the ATP-binding pocket of its kinase domain and plays a main role in cytoskeleton regulation. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: TNIK, 10ug (Human, recombinant; amino acids 1-367). MW: ~67kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT, MnCl2. TNIK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/23043/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.