ADP-Glo Kinase Assay + TRKB Kinase Enzyme System
Recombinant human TRKB (455-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKB is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TRKB is the high-affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations (1). TRKB is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway (2). Signaling through TRKB leads to cell differentiation. Mutations in the TRKB gene have been associated with obesity and mood disorders. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: TRKB, 10ug (Human, recombinant; amino acids 455-end). MW: ~67kDa. Substrate: Poly (4:1 Glu, Tyr) peptide. Other: Reaction Buffer, DTT, MnCl2. TRKB NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/4915/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.