ADP-Glo Kinase Assay + ABL1 Kinase Enzyme System
Recombinant human ABL1 (amino acids 27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion and stress response. Activity of ABL protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. Translocation and head-to-tail fusion of the BCR and ABL1 genes are present in many cases of chronic myelogenous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDK1-mediated phosphorylation, suggesting a cell cycle function for ABL1. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: ABL1, 10ug (Human, recombinant; amino acids 27-end). MW: ~135kDa. Substrate: Abltide (EAIYAAPFAKKK); derived from the C-terminus of ABL. Other: Reaction Buffer, DTT. ABL1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/25/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.