ADP-Glo Kinase Assay + ALK6 Kinase Enzyme System
Recombinant human ALK6 (BMPR1B) (149-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ALK6 (also known as BMPR1B) is a member of the transmembrane serine/threonine kinase that is the member of the bone morphogenetic protein (BMP) receptor, which is closely related to the activin receptors, ACVR1 and ACVR2. ALK6 is mainly involved in the endochondral bone formation and embryogenesis. ALK6 is expressed in normal and cancerous prostate tissues and used in the endocrine therapy given to prostate cancer patients (1). ALK6 receptor trafficking also plays a significant role in FOP pathogenesis and is used in human T-cell differentiation (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: ALK6, 10ug (Human, recombinant; amino acids 149-end). MW: ~68kDa. Substrate: TGFBR1 peptide (KKKVLTQMGSPSIRCS(pS)VS); derived from human SMAD3 (215-230). Other: Reaction Buffer, DTT. ALK6 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/658/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.