ADP-Glo Kinase Assay + Aurora B Kinase Enzyme System
Recombinant full-length human Aurora B was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. Aurora B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation. Aurora B localizes to the microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Aurora B inhibits the microtubule depolymerizing activity of mitotic centromere-associated kinesin (MCAK) by phosphorylating MCAK on Ser92. This phosphorylation also regulates MCAK translocalization from kinetochores to the centromere. Aurora B has been identified as a target for the development of new anticancer agents since inhibition of Aurora B gives rise to the more pronounced antiproliferative phenotype. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: Aurora B, 10ug (Human, recombinant full-length). MW: ~68kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. Aurora B NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/9212/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.