ADP-Glo Kinase Assay + BMX Kinase Enzyme System
Recombinant full-length human BMX was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The BMX gene encodes a non-receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells. The BMX gene is located on chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked agammaglobulinemia. BMX, is found to induce activation of the Stat signaling pathway. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: BMX, 10microg (Human, recombinant full-length). MW: ~110kDa. Substrate: Poly (4:1 Glu, Tyr) peptide. Other: Reaction Buffer, MnCl2, DTT. BMX NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/660/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.