ADP-Glo Kinase Assay + HIPK1 Kinase Enzyme System
Recombinant human HIPK1 (156-555) was expressed in Sf9 cells using an N-terminal GST tag. HIPK1 or homeodomain-interacting protein kinase 1 is a ser/thr protein kinase and a member of the HIPK family. HIPK 1 is a nuclear kinase that phosphorylates homeodomain transcription factors. HIPK1 phosphorylates DAXX, and this leads to its relocalization and subsequent decrease in transcriptional repression activity (1). HIPK1 also interacts with p53 and phosphorylates it on serine residues. HIPK1 expression is elevated in breast cancer cell lines and embryonic fibroblasts from HIPK1-null mice showing more susceptibility to apoptosis induced by DNA damage (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: HIPK1, 10ug (Human, recombinant; amino acids 156-555). MW: ~71kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. HIPK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/204851/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.