ADP-Glo Kinase Assay + p38 alpha Kinase Enzyme System
Recombinant full-length human p38 alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38 alpha (SAPK2A) is a member of the p38 MAPK family, which are activated by various environmental stresses and proinflammatory cytokines. The activation of p38 requires its phosphorylation by MAP kinase kinases (MKKs) or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of p38 include transcription regulators ATF2, MEF2C and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation as well as in genotoxic stress response. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: p38 alpha, 10ug (Human, recombinant full-length). MW: ~67kDa. Substrate: p38 peptide. Other: Reaction Buffer, DTT. p38 alpha NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1432/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.